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Archive for the ‘Bio-Rad Tutorial’ Category

An Advanced Look at Droplet Digital PCR

 :: Posted by American Biotechnologist on 12-07-2011

As a follow up to our video introduction to droplet digital PCR we are proud to present you with an advanced video presentation on droplet digital PCR technology. Please have a look and let us know what you think!

Video Introduction to Droplet Digital PCR

 :: Posted by American Biotechnologist on 12-05-2011

As a follow up to Bio-Rad’s acquisition of QuantaLife, we are proud to present the following educational training video: “An Introduction to Droplet Digital PCR”, given by award winning field application specialist Dr. Sean Taylor.

Be sure to stay tuned for the advanced training video which will be posted in the near future.

Introducing the 3rd Generation of PCR

 :: Posted by American Biotechnologist on 12-01-2011

QuantaLife, which was recently acquired by Bio-Rad Laboratories, produces an innovative droplet digital PCR system that provides quantification of target molecules with unprecedented precision and sensitivity. In the videos that follow, QuantaLife’s VP of Application Development Dr. Serge Saxonov, introduces Droplet Digital™ PCR and explains various aspects of the technology.

Protein blotting guide for novice and advanced users

 :: Posted by American Biotechnologist on 11-15-2011

Protein blotting is a staple technique of most molecular biology and proteomics laboratories. In previous posts, we discussed topics such as semi-dry protein transfer and protein transfer methods, and we even did a multi-part series on western blotting.

Now, we are proud to present you with a 43 page protein blotting guide put together by Bio-Rad Laboratories. The guide is organized into two parts which cover the theory and methods behind protein blotting. You will learn topics such as methods and instrumentation, the difference between various membranes and tranfer buffers, the ins and outs of transfer conditions, detection and imaging and a host of different blotting and detection protocols.

The guide is fairly technical and is appropriate for both novice and advanced users alike.

Click on the link to download the Protein Blotting Guide now.

Transfer Efficiency: Influence of the Gel Structure

 :: Posted by American Biotechnologist on 10-17-2011

With its proprietary transfer buffer, the Trans-Blot Turbo system generates very fast transfer even for high molecular weight proteins. However, as indicated previously, the gel composition, i.e. the acrylamide – bis-acrylamide network density, influences the transfer efficiency. A protein can more easily move out of the gel during the transfer if it is located in a portion of gel that has the widest pore structure. As proteins above 150 kD are always located on the first top part of a gel, the most efficient transfer of those large proteins is achieved when using gradient gel with a concentration of 4% of acrylamide – bis-acrylamide at the top of the gel. The transfer efficiency of proteins from an Any kD homogeneous acrylamide gel and a 4-20% gradient gel is illustrated.

Qualitative transfer efficiency comparison of HMW proteins on homogeneous acrylamide % and gradient gel. Precision Plus Protein™ Unstained standard and E. Coli homogenate (20 μg) were run on both Criterion TGX Any kD Stain-Free and 4-20% gels at 300V for 18 min. The total protein content was detected with the Stain-Free technology using the Gel-Doc EZ imaging system. The gels were then transferred with the Trans-Blot Turbo system with the 7 min preset program using the Trans-Blot Turbo PVDF transfer packs. The total protein content remaining in the gel is detected with the Stain-Free detection, using the same exposure parameters as used with the gels before the transfer for reliable comparison. The content of protein was also detected with the Stain-Free detection on the membrane. Even if most of the proteins are transferred in 7 min, the gradient gel contains less HMW proteins than the homogeneous gel after the transfer.