You are currently browsing the archives for the Bio-Rad Tutorial category.

Archive for the ‘Bio-Rad Tutorial’ Category

Increase Western Blot Throughput with Multiplex Fluorescent Detection

 :: Posted by American Biotechnologist on 01-30-2012

The most common method for analyzing protein expression levels is western blotting with detetion of a single protein target, using horseradish peroxidase-conjugated or alkaline phosphatase-conjugated antibody probes combined with colorimetric or chemiluminescent detection. While these methods work well for studying a single target, they are unsuitable for anlayzing multiple targets at the same time, particularly if the target proteins are of unknown or similar sizes. For analysis of multiple targets, the blot is typically stripped and reprobed for additional targets of interest. Reprobing is time consuming, and often some of the target protein on the blot is lost as a result of the stripping procedure. If one protein is removed to a greater of lesser extent relative to another protein, the ability to quantitate the relative amounts of diffferent proteins of interest is compromised.

In this technical note, you will be introduced to fluorescent western blotting detection which is superior to traditional western blotting when trying to analyze multiple proteins.

Advantages include:

  • fast and quantitative detection of multiple proteins in a single experiment
  • sensitivity compared to chemiluminescent detection
  • linear dynamic range up to 10 times greater than that of chemiluminescent detection
  • fewer experimental steps than chemiluminescent detection
  • no substrate requirement, and therefore no risk of exhausting the substrate and causing a “dead zone” in the blot
  • the ability to visualize and quantitate both phosphorylated and non-phosphorylated forms of individual proteins

The technical note is divided into three sections to help those who are new to fluorescent western blot detection quickly generate reliable and reproducible results.

Click here to download the the technote now!

Quantity One Imaging Software Video Tutorial

 :: Posted by American Biotechnologist on 01-04-2012

Learn how to use Quantity One Imaging Software from Bio-Rad Laboratories.

An Advanced Look at Droplet Digital PCR

 :: Posted by American Biotechnologist on 12-07-2011

As a follow up to our video introduction to droplet digital PCR we are proud to present you with an advanced video presentation on droplet digital PCR technology. Please have a look and let us know what you think!

Video Introduction to Droplet Digital PCR

 :: Posted by American Biotechnologist on 12-05-2011

As a follow up to Bio-Rad’s acquisition of QuantaLife, we are proud to present the following educational training video: “An Introduction to Droplet Digital PCR”, given by award winning field application specialist Dr. Sean Taylor.

Be sure to stay tuned for the advanced training video which will be posted in the near future.

Introducing the 3rd Generation of PCR

 :: Posted by American Biotechnologist on 12-01-2011

QuantaLife, which was recently acquired by Bio-Rad Laboratories, produces an innovative droplet digital PCR system that provides quantification of target molecules with unprecedented precision and sensitivity. In the videos that follow, QuantaLife’s VP of Application Development Dr. Serge Saxonov, introduces Droplet Digital™ PCR and explains various aspects of the technology.