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Archive for the ‘Bio-Rad Tutorial’ Category

Chromatography 101: An Introduction to Medium Pressure Chromatography

 :: Posted by American Biotechnologist on 07-24-2013

Chromatography 101: An Introduction to Ion Exchange Chromatography

 :: Posted by American Biotechnologist on 07-11-2013

Video Tutorial: Quantitative Western Blotting

 :: Posted by American Biotechnologist on 06-17-2013

Are you getting the most from your western blot data? Does Imaging technique matter? Film or Imager? In this video, we describe a methodology to obtain reliable quantitative data from chemiluminescent western blots using standardization procedures coupled with the updated reagents and detection methods. For the best resolution, watch the video in full screen at HD resolution.

Teaching Real Time PCR In the Classroom

 :: Posted by American Biotechnologist on 10-18-2012

Recorded from a live webinar.

What the heck is a coefficient of variation?

 :: Posted by American Biotechnologist on 06-25-2012

Statistics is probably one of the most important tools you will use in your research career. Stats have the ability to turn your seemingly beautiful data into a pile of useless junk, or to convert questionable data into an award winning publication.

Like many molecular biologists, I am statistically challenged. I’ve tried and tried again to learn the meaning behind important terms such a “p-value,” “ANOVA test” and “correlation coefficient” all to no avail. I am a stats flunkie. When I asked my supervisor about my learning disability he told me that I am a molecular biologist and that really there is not hope.

The best we can shoot for is p<0.05 and call it significant.

For many years, I was resigned to the fact that I’d never understand stats and that’s why the position of in-house statistician existed. To help, (if not to make fun of), people like me. Fortunately, there is new hope. Bio-Rad Laboratories has now produced a series of video tutorials geared towards helping those who have less fortunate statistical skills.

In the video below, Bio-Rad experts explain how to calculate a coefficient of variation. I found the video to be pretty clear and it has somewhat clarified this murky term for me. What about you?