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How to Avoid Unstable Results When Performing High-Throughput qPCR

 :: Posted by American Biotechnologist on 04-28-2010

If your biotechnology research requires you to be engaged in high-throughput Quantitative PCR analysis than you’ve probably made good use out of automated systems such as liquid handling robots and plate stackers. And what a relief they are. Imagine what your poor thumb would feel like if you had to pipette out tens of thousands of reactions. Or think about how much time you would have wasted standing around waiting for a run to end in order to load the next plate into the QPCR machine.

While automated handing systems are fantastic, they also bring with them the complicated question of sample stability. When samples are loaded into the automated system, they may be stored there at room temperature for hours. As such, it is important for the reaction mix to be stable at room temperature for the duration of the waiting period.

In this Bio-Rad Technical note, you will learn how Bio-Rad’s SsoFast EvaGreen supermix meets the challenge of providing reaction mix stability and can be used to acheive consistent results over a large dynamic range of templates with automated runs lastng up to 48 hours.

Stability for Automated qPCR

Faster, Cleaner and More Specific Affinity Tag Purification

 :: Posted by American Biotechnologist on 04-22-2010

Protein overexpression and affinity purification are used in various biotechnology research fields and play an important role in the post genomic era. While many of the common purification techniques make use of tags like 6XHis and GST, they face drawbacks such as significant background and an extra tag-removal step (especially if the tag is known to interfere with the protein’s function).

Bio-Rad’s Profinity eXact protein purification system utilizes an immobilized engineered protease that specifically recognizes and binds the Profinity eXact tag with high affinity. Following purification, the protein is washed and the tag is cleaved while still on-column.

This technical note demonstrates how the Profinity eXact purification system overcomes concerns common to expression and purification systems in eukaryotic cells including: poor tag expression, tag degradation, posttranslational modification and misfolding of tag and excessive background contamination. The results indicate that the Profinity eXact system can be used in eukaryotic cells for single-step purification of tag-free proteins with low background and without compromising the obvious function of the system.

Faster, Cleaner, More Specific Affinity Tag Purification

Perform Efficient Electroporation with Bio-Rad’s MXCell and Save Time, Money and Effort

 :: Posted by American Biotechnologist on 04-01-2010

As discussed in an earlier post on siRNA delivery, Bio-Rad’s Gene Pulser MXCell Electroporator is a powerful tool for biotechnology labs involved in delivering siRNA into difficult to transfect cells. In this technical note, biotechnology experts from the University of Rochester demonstrate how the MXCell can be used to simultaneously optimize electroporation conditions in multiple cell types thereby saving time, reagents and effort.

Electroporation of Smooth Muscle Cells Using the Gene Pulser MXcell Electroporation System

Delivering siRNA into Difficult to Transfect Cells Using Bio-Rad’s MXCell Electroporator

 :: Posted by American Biotechnologist on 03-31-2010

Small interfereing RNA (siRNA) technology is a powerful tool for facilitating post-transcriptional gene silencing. As with most transfection techniques, siRNAs have been traditionally delivered into the cell by one of four transfection techniques: lipid-mediated, chemical-mediated, viral transfection or electroporation.

In this paper, a team from the Beckman Research Institue of the City of Hope demonstrate how Bio-Rad’s MXCell electroporator can be used to optimize transfection conditions for B-Cell lymphomas which are extremely difficlut to transfect by most traditional methods.

Delivering siRNA into Difficult to Transfect Cells Using Bio-Rad’s Gene Pulser MXcell Electroporation System

Using Bio-Rad’s ProteOn XPR36 for Antibody Characterization and Development

 :: Posted by American Biotechnologist on 03-25-2010

The Bio-Rad ProteOn XPR36 is a surface plasmon resonance (SPR) system capable of analyzing 36 protein-protein interactions in one experiment. Up to 6 antibodies may be simultaneously immobilized in parallel channels and assayed with panels of up to 6 antigens.

The Antibody Characterization and Development Using the Bio-Rad ProteOn technical note describes how the ProteOn can be used for:

  • Hybridoma screening and ranking
  • Epitope mapping
  • Kinetic analysis of antibody-antigen specificity
  • Determination of cross-reactivity of antibodies used in multiplex assays
  • For a great primer on Bio-Rad’s ProteOn XPR36 check out this brief video tour