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Archive for the ‘qPCR tutorial’ Category

Quantitative PCR (qPCR): Hallmarks of an Optimized SYBR Green I Assay

 :: Posted by American Biotechnologist on 02-16-2010

If you are currently doing quantitative PCR (qPCR) or plan on beginning qPCR experiments you MUST check out this latest video tutorial from Bio-Rad’s tech support group.

This tutorial describes the characteristics of an optimized SYBR green I quantitative PCR (qPCR) assay. qPCR assays must be optimized to ensure results that are biologically and statistically significant. Topics include a brief review of qPCR chemistry, with an emphasis on SYBR Green I reactions, and definitions of the four main characteristics, or hallmarks of an optimized qPCR assay.

qpcr syber tile

Real-Time Quantitative PCR Data Analysis Tutorial

 :: Posted by American Biotechnologist on 11-12-2009

Bio-Rad Laboratories has put together a great tutorial on QPCR analysis including a thorough review of what the graphs mean and an overview of Absolute and Relative methods of quantification. The tutorial also includes a great explanation of the differences between Livak, delta CT and the Pfaffl methods of analyzing your results.

Watch part II: normalization against a reference gene