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Archive for the ‘Droplet Digital PCR’ Category

News: Droplet Digital PCR Improves Detection Sensitivity of Telomerase Activity Assay

 :: Posted by American Biotechnologist on 04-10-2013

Researchers from the University of California, San Francisco, and Bio-Rad have demonstrated that Bio-Rad’s Droplet Digital PCR (ddPCR) technology can dramatically improve the sensitivity, precision, and throughput of a popular assay for telomerase activity.

Currently, researchers are investigating telomerase activity as a biomarker for cancer diagnosis and as a target for anticancer drugs. Measuring it’s activity more sensitivity may enhance our understanding of it’s role in diseases.

This research was presented at the American Association of Cancer Research (AACR) Annual Meeting on Tuesday, April 9.

You can view the press release here:

What can be more accurate than real time PCR?

 :: Posted by American Biotechnologist on 08-01-2012

“People are different from each other in ways that are fascinating and medically important, and to understand the ways in which variations in our genome give rise to those differences presents an important and interesting set of questions,” says Dr Steve McCarroll, faculty member and principal investigator with the Department of Genetics at Harvard Medical School and the Broad Institute of Harvard and MIT. The McCarroll laboratory studies the biological effects of human genome polymorphism, seeking to define how genome variation influences gene expression and risk of disease. Their work requires them to accurately measure the copy number of a genomic sequence with integer precision in hundreds of people. While real-time PCR and CGH (comparative genomic hybridization) arrays are useful for measuring simple deletions and duplications, these techniques simply weren’t sufficient for obtaining single-molecule resolution of target sequences with extreme precision and accuracy. That’s where Droplet Digital PCR entered the story.

Learn how Dr. McCaroll’s lab, along with other labs across the globe utilize ddPCR in applications such as:

  • Absolute quantification of HIV proviral DNA and human genomic DNA in the same reaction
  • Absolute quantification of HIV viral RNA using two-step reverse transcription-ddPCR
  • Rare event detection of BRAF V600E, EGFR L858R and T790M, JAK-2, and cKIT (cancer mutation analysis and drug resistance mutation)
  • Absolute quantification of miRNA from circulating nucleic acids for biomarker identification
  • High-resolution copy number variation (CNV) analysis of human and mouse DNA’s
  • High-resolution CNV analysis of the MET gene, association study for lung cancer
  • Absolute quantification of next generation sequencing library
  • Rare event detection, mitochondrial mutagenesis
  • Absolute quantification of the BCR-ABL (Philadelphia chromosome) fusion gene and transcript, association with leukemia
  • Absolute quantification and sex determination of fetal DNA in circulating nucleic acid purified from maternal blood

For more information read “Third Generation PCR: A Novel Way to Accurately Measure Copy Number Variation in the Human Genome.”

How Droplet Digital PCR is Helping to Cure HIV

 :: Posted by American Biotechnologist on 07-23-2012

Timothy Ray Brown is the only person who has been cured of HIV to date (Pollack 2011). Dubbed the Berlin Patient, the now 46-year-old Brown was living with HIV and leukemia in Berlin, Germany when he received two bone marrow stem cell transplants from a donor who carried, in both copies of his CCR5 gene, a well-characterized natural defect, the CCR5-∆ 32 mutation. (CCR5 is a major co-receptor used by HIV to enter and infect CD4+ cells.) The CCR5-∆ 32 mutation rendered the CCR5 protein inactive and thus made the donor’s cells naturally resistant to HIV. After completing the transplants in 2007 and 2008, Brown immediately stopped taking antiviral drugs and has remained HIV-free ever since, igniting considerable interest within the cure research community.

Find out how Droplet Digital™ PCR played a role in Timothy’s recovery.

Read more…

Great video explanation of droplet digital PCR

 :: Posted by American Biotechnologist on 03-21-2012

The QX100 Droplet Digital PCR system from Bio-Rad Laboratories is the third generation of PCR technology. The system provides an absolute measure of target DNA molecules with unrivaled performance in precision, accuracy, and sensitivity for quantitative PCR applications. Watch the video below to learn more about this powerful technology.

Related posts:
Introducing the 3rd Generation of PCR
Video Introduction to Droplet Digital PCR
An Advanced Look at Droplet Digital PCR

An Advanced Look at Droplet Digital PCR

 :: Posted by American Biotechnologist on 12-07-2011

As a follow up to our video introduction to droplet digital PCR we are proud to present you with an advanced video presentation on droplet digital PCR technology. Please have a look and let us know what you think!