Archive for the ‘Droplet Digital PCR’ Category
Since its introduction two years ago, Droplet Digital PCR has transformed multiple fields of research, including infectious disease, cancer biomarker analysis, and genomic variation analysis. In this seminar, Dr. David Dodd describes the application of ddPCR in his research studies with Prof. David Corey and colleagues at UT Southwestern Medical Center at Dallas. The Corey Lab focuses on using antisense oligonucleotides and duplex RNAs to control gene expression in human cancer cells.
Clearly digital PCR has come a long way in recent years, thanks in large measure to the development of commercial systems like the QX200. These technology advances seem to indicate a tipping point where a greater number of researchers will soon have access to the technology, which will spur development of new applications that take advantage of the full capabilities of digital PCR and move scientists towards more robust biomarker studies and even single cell analyses.
Could the next revolution in PCR be digital? In an article appearing in Biotechniques, Nathan Blow takes a look at the history of digital PCR and why the methodology might have finally reached a tipping point in development.
Read the article to find out:
- What makes digital PCR different from traditional PCR
- A short history of digital PCR
- Why did it take so long for the technology to catch on with developers and researchers?
- How does digital PCR compare to real-time-quantitative PCR?
To learn more read Digital PCR: Separating from the Pack.
Droplet Digital PCR (ddPCR™) enables accurate, precise, and sensitive quantification of specific nucleic acid sequences. In addition to the standard detection of two targets using two different fluorophores, it is possible to increase the number of targets detected by varying parameters that affect PCR efficiency and end-point fluorescence. In this case, we describe a method to multiplex assays by varying the concentrations of primers and probes or the type of fluorophores used. This allows users to expand the number of simultaneously detected targets up to four. Increasing the number of potential targets per test is a significant improvement for ddPCR, dramatically augmenting the information output of each sample.