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Archive for the ‘Bio-Rad Product Highlight’ Category

Improving Visibility of qPCR Reaction Mixtures

 :: Posted by American Biotechnologist on 05-14-2014

Bio-Rad Laboratories recently announced the launch of its Precision Blue real-time PCR dye, making it easier for researchers to set up real-time PCR (qPCR) experiments.

The blue dye improves the visibility of qPCR reaction mixtures, enabling loading of small volume reactions with greater speed, accuracy, and precision. Applications such as single nucleotide polymorphism (SNP) genotyping and high-throughput gene expression analysis using 384-well plates will particularly benefit from use of this dye.

Precision Blue dye is compatible with Bio-Rad’s universal qPCR supermixes (iTaq™ universal and SsoAdvanced™ universal supermixes) and does not interfere with SYBR® Green- or probe-based detection. The dye can be used with all Bio-Rad CFX real-time PCR systems and all other vendors’ real-time PCR systems.

For convenience, Precision Blue can be added directly to the supermix or used as a component of a reaction master mix. The dye is concentrated 200x, and delivers 500 x 20 µl reactions.

For more information about Precision Blue dye, please visit www.bio-rad.com/PrecisionBlue.

Staining of Live Cells for Multicolor Flow Cytometry Experiments

 :: Posted by American Biotechnologist on 05-07-2014

Cytotrack_productsBio-Rad Laboratories, Inc. today announced the launch of its CytoTrack Cell Proliferation Assays that provide unmatched flexibility when designing a multicolor flow cytometry experiment, allowing researchers to efficiently stain and track live cells in four colors for up to 10 generations — two more generations than other currently available methods can follow.

CFDA-SE, the most commonly used reagent for monitoring cell proliferation, is difficult to use in multicolor experiments because it has the same spectra as green fluorescent protein and the most frequently used fluorophore, fluorescein.

Unlike CFDA-SE and other popular cell proliferation assays that are available in only one color, Bio-Rad’s CytoTrack Assays are available in four different emission wavelengths ranging from blue to far red. This allows researchers to choose the one most suitable for their multicolor flow cytometric experiment.

The CytoTrack Assay dye reacts with primary amines using a proprietary chemistry that provides effective labeling of cells without the large efflux usually seen with CFDA-SE.

“This unique feature allows researchers to easily visualize up to 10 cell divisions, making it ideal for those interested in studying the induction and inhibition of cell division in any in vitro experimental model,” said Mary Thao, Bio-Rad Product Manager in the Gene Expression Division.

CytoTrack Assays come in easy-to-use vials with material sufficient for 50 experiments. The dye can be added directly into the culture medium, allowing researchers to save time.

Bio-Rad offers additional reagents and consumables to complete each step of flow cytometry experiments, such as cell viability assays, primary antibodies, and antibody labeling kits.

For more information about Bio-Rad’s cell proliferation assays, please visit bio-rad.com/cellproliferation

Bio-Rad Laboratories Awarded Best New Life Sciences Product of 2013

 :: Posted by American Biotechnologist on 05-01-2014

SelectScience was delighted to announce the QX200™ Droplet Digital™ PCR System, by Bio-Rad, as the winner of the Scientists’ Choice Award for Best New Life Sciences Product of 2013. The award was presented to Bio-Rad at the American Association for Cancer Research (AACR) Annual Meeting 2014.

Click on the photo below to navigate to the SelectScience website and view the video.

select science ddpcr award

Transforming Cancer Research: Droplet Digital™ PCR Plays Integral Role at Fred Hutchinson Cancer Research Center

 :: Posted by American Biotechnologist on 04-15-2014

It’s no surprise that Droplet Digital PCR (ddPCR) has found its place in the world of cancer research. Bio-Rad’s ddPCR™ technology has a remarkable ability to quantify miniscule amounts of target DNA and RNA, and thus can contribute to early detection of rare tumorigenic mutations against a high background of “normal” DNA as well as to other applications, including identifying cancer subtypes, optimizing drug treatment plans, and studying tumor evolution. At the Fred Hutchinson Cancer Research Center in Seattle, scientists are producing some of the most important advances in prevention, early detection and treatment of cancer and other diseases. They are studying the disease process from every angle seeking to uncover factors that influence a person’s likelihood of getting cancer. Understanding of such factors, of course, can help reduce risk and save lives. To learn more about how Droplet Digital PCR is being wielded to fight against cancer, we caught up with two researchers – Muneesh Tewari and Jason Bielas— who are using Bio-Rad’s QX100™ Droplet Digital™ PCR system in their quest to break through the current limits of nucleic acid detection and quantification.

Read more…

Learn the Basics of Image Lab Software

 :: Posted by American Biotechnologist on 04-08-2014

Image Lab™Software: Learn the Basics from the Experts

Join Bio-Rad Laboratories for a 30 minute live webinar developed and delivered by Ben Wang, PhD, Senior Technical Support Specialist, Bio-Rad Laboratories

The webinar will take place TOMORROW – Wednesday April 9, 2014 at 9:00 AM Pacific.

As you get ready to use your new system, Bio-Rad will provide you with an opportunity to learn the basic features of Image Lab Software. This training will cover the primary tools, as well as how to acquire an image.

The webinar will be recorded and the playback link will be shared with registrants

Click here to register for the webinar.