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Archive for the ‘Bio-Rad Product Highlight’ Category

Learn the Basics of Image Lab Software

 :: Posted by American Biotechnologist on 04-08-2014

Image Lab™Software: Learn the Basics from the Experts

Join Bio-Rad Laboratories for a 30 minute live webinar developed and delivered by Ben Wang, PhD, Senior Technical Support Specialist, Bio-Rad Laboratories

The webinar will take place TOMORROW – Wednesday April 9, 2014 at 9:00 AM Pacific.

As you get ready to use your new system, Bio-Rad will provide you with an opportunity to learn the basic features of Image Lab Software. This training will cover the primary tools, as well as how to acquire an image.

The webinar will be recorded and the playback link will be shared with registrants

Click here to register for the webinar.

Designing the Perfect Quantitative Western Blot

 :: Posted by American Biotechnologist on 04-02-2014

A methods article published yesterday provides a rigorous and concise workflow with specific instructions on how to produce and analyze quantitative data using western blot experiments. The paper, coauthored by Bio-Rad scientists and published in BioMed Research International, also highlights recently introduced technologies that improve reproducibility. The result is a powerful, step-by-step guide to obtaining quantitative and reproducible densitometric data from western blots regardless of the specific experiment.

Although western blotting is a well-established laboratory technique, it has recently come under fire as a quantitative method because extreme care must be taken when generating and interpreting the resulting data.

The technique is challenging and requires following a rigorous methodology to achieve reproducible and quantitative data. According to a recent survey of more than 750 labs, 41% of researchers say their western blots fail a quarter of the time.

Dr. Aldrin Gomes, an assistant professor at University of California, Davis, agrees that flawed western blots are not unusual. To compare expression of a protein of interest from sample to sample, protein abundance is commonly normalized to a housekeeping gene. “When I see a large, dense band for the protein of interest or the housekeeping protein, I cringe,” says Gomes. That dense band usually means the protein of interest or housekeeping protein was no longer within the assay’s linear dynamic range. No accurate quantitative data can be extracted from such blots.

Sean Taylor discusses the BioMed Research International paper he coauthored.

Another common reason for failure of quantitative western blots is flawed or incomplete protocols, according to Sean Taylor, the paper’s lead author and a Bio-Rad field application scientist (watch a video of him discussing the paper on the left). To address this, Taylor’s review pays special attention to experimental design and sample preparation and discusses proper definition of the linear dynamic range of protein loading, all key factors for generating meaningful quantitative western blot data.

Taylor also introduces more advanced concepts to improve reproducibility, simplify workflow, and reduce the time and cost of western blotting. One such technique is stain-free total protein normalization, which over the past year has proven superior to using housekeeping proteins or total protein staining to correct for loading errors.

With this article, Taylor hopes researchers now have a simple guide to ensure quantitative and reproducible western blot data for all research fields that rely on this technique.

To read the open access research article, visit http://bit.ly/1kCAOcr.

For additional resources please consult Bio-Rad’s guide to Troubleshooting Western Blots.

Droplet Digital™ PCR for Gene Expression and MicroRNA Analysis

 :: Posted by American Biotechnologist on 03-31-2014

An Ode to E. Coli

 :: Posted by American Biotechnologist on 03-26-2014

A Simple Way to Label Your Own Antibodies

 :: Posted by American Biotechnologist on 03-13-2014

Bio-Rad Laboratories announced the launch of its ReadiLink antibody labeling kits, one of the market’s simplest antibody conjugation solution for labeling microscale amounts (50–100 µg) of antibody. These kits are ideal for researchers interested in labeling their own antibodies for flow cytometry and cell sorting applications.

Using the ReadiLink antibody labeling kits, researchers can label their antibodies in two easy steps. The protocol takes only 70 minutes, and the labels are as bright and photostable as traditional dyes. Bio-Rad offers 10 different fluorophores whose excitation/emission wavelengths range from UV to infrared.

ReadiLink antibody labeling kits permit fluorophore conjugation of antibodies in two simple steps.

“The labeling kits will benefit a wide range of researchers,” said Mary Ferrero, Bio-Rad Product Manager in the Gene Expression Division, Life Sciences Group. “ReadiLink antibody labeling kits are ideal for researchers who are using a rare antibody, are interested in an antibody that is not commercially available with the appropriate fluorophore, or who want to label an antibody with a fluorophore that will fit into their multicolor flow cytometric experiment.”

Bio-Rad also offers additional reagents and consumables for each step of flow cytometry experiments, such as the ReadiDrop™ cell viability assays and primary antibodies.

For more information about Bio-Rad’s antibody labeling kits, please visit www.bio-rad.com/antibodylabeling.