One of our favorite techniques to discuss on the American Biotechnologist is quantitative real-time PCR (qPCR). Previous posts on the topic include:
- a qPCR Data Analysis Tutorial
- Hallmarks of an optimized SYBR Green I assay
- Avoiding unstable results when performing qPCR
- Tutorial on validating a SYBR Green I assay
- Becoming a real time qPCR expert
- qPCR analysis: it’s what’s inside that counts
- a practical approach to MIQE for the bench scientist
That is why we are excited to tell you about Biomed Central’s (BMC) topical series on Quantitative Real Time PCR normalization and optimization, Edited by Joshua S. Yuan. According to BMC, topical series bring together manuscripts published in BMC Research Notes that are associated with individual topics. Through the topical series, BMC Research Notes aims to highlight exciting topical areas of research and provide a home for concise articles that raise awareness and encourage discussion of the subjects covered.
I’ve scanned through some of the articles posted on BMC’s website and several of them (such as RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts) have piqued my interest. I hope to review some of these articles in this forum over the coming weeks and I welcome your comments and feedback.